Reporter

Part:BBa_K577882:Design

Designed by: Alberto Purwada   Group: iGEM11_BU_Wellesley_Software   (2011-09-11)


pC+AraC+pBad+RFPc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1833
    Illegal AgeI site found at 1945
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

The RBS, RFP, and Terminator were taken from BBa_J61002 using SpeI and PstI restriction enzymes.


Source

The part is obtained from ligating several biobricks.

References